Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: SNRPB/CCNB1 axis promotes hepatocellular carcinoma progression and cisplatin resistance through enhancing lipid metabolism reprogramming
doi: 10.1186/s13046-025-03463-y
Figure Lengend Snippet: Functional analysis of SNRPB knockdown in HCC cells. ( A ) Validation of SNRPB knockdown efficiency in SK-HEP-1 and HCCLM3 cells at both mRNA and protein levels using two effective shRNAs (shSNRPB-2 and shSNRPB-3). ( B ) CCK-8 assay demonstrates the inhibitory effects of SNRPB knockdown on HCC cell proliferation in SK-HEP-1 and HCCLM3 cells. ( C ) Colony formation assay shows that SNRPB knockdown significantly suppresses the clonogenic capacity of HCC cells. ( D ) Flow cytometry analysis reveals that SNRPB knockdown significantly increases apoptosis rates in SK-HEP-1 and HCCLM3 cells. ( E ) Transwell assay demonstrates that silencing SNRPB significantly reduces the migratory capacity of HCC cells. ( F ) The pictures taken from the excised xenografts of indicated experimental groups. ( G ) Tumor volume measurements in subcutaneous xenograft tumor models showing the effects of SNRPB knockdown on tumor growth. ( H ) Tumor weight analysis at the time of sacrifice in xenograft models. ( I ) IHC staining of tumor tissues showing Ki67 expression levels. Data were drawn as mean ± SD ( n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001
Article Snippet: The assessment of cell migration was conducted using a Transwell chamber (Corning).
Techniques: Functional Assay, Knockdown, Biomarker Discovery, CCK-8 Assay, Colony Assay, Flow Cytometry, Transwell Assay, Immunohistochemistry, Expressing